Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of SP and the recovery rates were calculated by comparing the measured value to the expected amount of SP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
92
EDTA plasma(n=5)
87-100
92
heparin plasma(n=5)
86-100
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-104%
89-101%
89-103%
EDTA plasma(n=5)
85-101%
83-96%
83-99%
heparin plasma(n=5)
81-100%
95-100%
86-99%
Sensitivity
< 4.688pg/ml
Notes
For Research Use Only
UniProt ID
P06767
Antigen
SP
Applications
ELISA
Applications Notes
SP ELISA Kit allows for the in vitro quantitative determination of SP concentrations in serum, plasma, tissue homogenates and other biological fluids.