Rat soluble Cluster of differentiation 86,sCD86 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08543r

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

soluble Cluster of differentiation 86,sCD86

Recovery

The recovery of mouse FGF10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	98-108
EDTA plasma (n=4)	95	90-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGF10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	102
	Range %	97-107
1:4	Average %	89
	Range %	86-92
1:8	Average %	94
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O35531

Applications	ELISA
Research Area	B72, sCD86

Storage	Store at 2-8°C. Please refer to protocol.

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