Rat SMOC1 ELISA Kit PicoKine®

Mfr.No.

EK1937

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.104	0.148	0.189	0.261	0.456	0.762	1.223	1.982

Description

Rat SMOC1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Smoc1 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

SPARC-related modular calcium binding protein 1; SPARC-related modular calcium-binding 1; Smoc1; smoc1

Immunogen

Expression system for standard: NS0; Immunogen sequence: H26-V452

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat SMOC1

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	159	688	1452	168	675	1526
Standard deviation	7.63	39.22	66.79	8.57	39.83	97.66
CV (%)	4.8%	5.7%	4.6%	5.1%	5.9%	6.4%

Protein Function

Lacks conserved residue (s) required for the propagation of feature annotation.

Background

This gene is mapped to 6q24. Human homolog binds calcium and may play a role in the extracellular matrix.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	159	162	177	168	167	5.49	3.3%
Sample 2	688	757	693	684	706	14.11	2.0%
Sample 3	1452	1596	1441	1365	1464	83.42	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	314280
Gene Name	Smoc1
UniProt ID	Q6IE50

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001454	EK1937	96 wells/kit	$750		Add to Cart

Rat SMOC1 ELISA Kit PicoKine®

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