Matrices listed below were spiked with certain level of Smad1 and the recovery rates were calculated by comparing the measured value to the expected amount of Smad1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-104
99
EDTA plasma(n=5)
85-102
95
heparin plasma(n=5)
93-103
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Smad1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Smad1/MADH1(Mothers Against Decapentaplegic Homolog 1)/BSP1/BSP-1/hSMAD1/JV4-1SMAD, mothers against DPP homolog 1(Drosophila)
Notes
For Research Use Only
UniProt ID
P97588
Antigen
Smad1
Applications
ELISA
Applications Notes
Smad1 ELISA Kit allows for the in vitro quantitative determination of Smad1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Smad1(Mothers Against Decapentaplegic Homolog 1) ELISA Kit