Rat Sirt2(NAD-dependent deacetylase sirtuin-2) ELISA Kit

Mfr.No.

ER0299

Alternative Names

Sirt2 ELISA Kit, SIR2L2 ELISA Kit, sirtuin 2 ELISA Kit, sirtuin type 2 ELISA Kit, NAD-dependent deacetylase sirtuin-2 ELISA Kit, SIR2-like protein 2 ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

0.313-20ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Sirt2 and the recovery rates were calculated by comparing the measured value to the expected amount of Sirt2 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-96	90
EDTA plasma(n=5)	87-98	93
heparin plasma(n=5)	85-104	92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sirt2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	91-103%	88-105%	88-105%
EDTA plasma(n=5)	88-101%	83-98%	85-100%
heparin plasma(n=5)	80-96%	87-100%	83-99%

Sensitivity

< 0.188ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Sirt2/SIR2L2/sirtuin 2/sirtuin type 2/NAD-dependent deacetylase sirtuin-2/SIR2-like protein 2

Notes

For Research Use Only

UniProt ID	Q5RJQ4
Antigen	SIRT2

Applications	ELISA
Applications Notes	Sirt2 ELISA Kit allows for the in vitro quantitative determination of Sirt2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10805827	ER0299	96T	$400		Add to Cart

Rat Sirt2(NAD-dependent deacetylase sirtuin-2) ELISA Kit

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