Rat SIRT1(NAD-dependent protein deacetylase sirtuin-1) ELISA Kit

Mfr.No.

ER1338

Alternative Names

SIRT1 ELISA Kit, SIR2alpha ELISA Kit, SIR2L1 ELISA Kit, SIR2-like protein 1 ELISA Kit, EC 3.5.1 ELISA Kit, S. cerevisiae ELISA Kit, homolog 1 ELISA Kit, sirtuin 1 ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of SIRT1 and the recovery rates were calculated by comparing the measured value to the expected amount of SIRT1 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	88-98	93
EDTA plasma(n=5)	86-99	91
heparin plasma(n=5)	86-103	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	88-95%	89-103%	87-104%
EDTA plasma(n=5)	89-101%	84-93%	87-99%
heparin plasma(n=5)	83-91%	80-100%	80-90%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

SIRT1/SIR2alpha/SIR2L1/SIR2-like protein 1/EC 3.5.1/S. cerevisiae, homolog) 1/sirtuin 1

Notes

For Research Use Only

UniProt ID	A0A0G2JZ79
Antigen	SIRT1

Applications	ELISA
Applications Notes	SIRT1 ELISA Kit allows for the in vitro quantitative determination of SIRT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10802340	ER1338	96T	$400		Add to Cart

Rat SIRT1 (NAD-dependent protein deacetylase sirtuin-1) ELISA Kit

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