Matrices listed below were spiked with certain level of Sigmar1 and the recovery rates were calculated by comparing the measured value to the expected amount of Sigmar1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
23-90
60
EDTA plasma(n=5)
21-104
60
heparin plasma(n=5)
4-96
41
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sigmar1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
1-98%
12-87%
13-92%
EDTA plasma(n=5)
7-87%
26-86%
0-70%
heparin plasma(n=5)
5-92%
1-84%
10-78%
Sensitivity
< 46.9pg/ml
Notes
For Research Use Only
UniProt ID
Q9R0C9
Applications
ELISA
Applications Notes
Sigmar1 ELISA Kit allows for the in vitro quantitative determination of Sigmar1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Sigmar1 (Sigma non-opioid intracellular receptor 1) ELISA Kit