Matrices listed below were spiked with certain level of SH3BGRL3 and the recovery rates were calculated by comparing the measured value to the expected amount of SH3BGRL3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
97
EDTA plasma(n=5)
87-100
94
heparin plasma(n=5)
89-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SH3BGRL3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-102%
90-104%
97-102%
EDTA plasma(n=5)
86-99%
83-96%
93-98%
heparin plasma(n=5)
82-97%
81-95%
82-94%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
B2RZ27
Applications
ELISA
Applications Notes
SH3BGRL3ELISA Kit allows for the in vitro quantitative determination of SH3BGRL3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat SH3BGRL3(SH3 domain-binding glutamic acid-rich-like protein 3)ELISA Kit