Rat Sh3 and Multiple Ankyrin Repeat Domains Protein 1

Mfr.No.

E2516Ra

Alternative Names

GKAP/SAPAP-interacting protein;SH3 and multiple ankyrin repeat domains protein 1;Shank1;Shank1a;Somatostatin receptor-interacting protein;Spank1;SPANK-1;SSTR-interacting protein;Sstrip;Synamon

Reactivity

Rat

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

226335

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.13ng/L

Background

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Overexpression promotes maturation of dendritic spines and the enlargement of spine heads via its ability to recruit Homer to postsynaptic sites, and enhances presynaptic function.

Gene ID	78957
UniProt ID	Q9WV48
Antigen	Shank1

Research Area

Neuroscience

Protocol

These standard curves of E2516Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.153	2.222	2.184
	2.29
1200ng/L	1.688	1.605	1.567
	1.522
600ng/L	1.003	1.057	1.019
	1.11
300ng/L	0.739	0.732	0.694
	0.725
150ng/L	0.405	0.406	0.368
	0.406
0ng/L	0.038	0.038	0
	0.038

Precision

We measured random samples of E2516Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1325.94	76.33	5.76
Intra-Assay	2	18	373.34	30.87	8.27
Intra-Assay	3	18	189.7	15.47	8.15

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115421	E2516Ra	48T	$320		Add to Cart
BHE12115421	E2516Ra	96T	$458		Add to Cart

Rat Sh3 and Multiple Ankyrin Repeat Domains Protein 1 - SHANK1 ELISA Kit

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