Rat sex hormone-binding globulin (SHBG) ELISA kit

Rat sex hormone-binding globulin (SHBG) ELISA kit

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Mfr.No.

CSB-E12118r

Sensitivity

375 ng/mL

Detection Range

375 ng/mL-6000 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

sex hormone-binding globulin, ABP, MGC126834, MGC138391, SBP, TEBG, androgen binding protein, sex steroid-binding protein, testis-specific androgen-binding protein, testosterone-binding beta-globulin, testosterone-estradiol-binding

Recovery

The recovery of human N-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-105
EDTA plasma (n=4)	97	90-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human N-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-100
1:2	Average %	97
	Range %	91-104
1:4	Average %	97
	Range %	92-108
1:8	Average %	94
	Range %	85-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average	Corrected
400	2.152	2.174	2.163	2.005
200	1.832	1.824	1.828	1.670
100	1.220	1.251	1.236	1.078
50	0.926	0.974	0.950	0.792
25	0.574	0.543	0.559	0.401
12.5	0.406	0.426	0.416	0.258
6.25	0.335	0.312	0.324	0.166
0	0.152	0.164	0.158	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P08689

Applications	ELISA
Research Area	SHBG

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508632	CSB-E12118r	96T	$790	Add to Cart
BHE10508632	CSB-E12118r	5x96T	$3792	Add to Cart
BHE10508632	CSB-E12118r	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.