Rat Serum amyloid A,SAA ELISA Kit

Mfr.No.

CSB-E08590r

Alternative Names

Serum amyloid A,SAA

Reactivity

Rat

Detection Range

78 ng/mL-5000 ng/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human RAB10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-105
EDTA plasma (n=4)	92	86-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human RAB10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-99
1:2	Average %	95
	Range %	90-99
1:4	Average %	92
	Range %	87-98
1:8	Average %	97
	Range %	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2500	2.186	2.230	2.208	2.041
1250	1.724	1.619	1.672	1.505
625	1.223	1.165	1.194	1.027
312.5	0.744	0.753	0.749	0.582
156.25	0.516	0.503	0.510	0.343
78	0.368	0.378	0.373	0.206
39	0.259	0.250	0.255	0.088
0	0.166	0.168	0.167	?

Species

Rattus norvegicus

Sensitivity

19.5 ng/mL

Limit Of Detection

450 nm

Antigen	serum amyloid A,SAA

Applications

ELISA

Research Area

SAA

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Human oral isolate Lactobacillus fermentum AGR1487 induces a pro-inflammatory response in germ-free rat colons. Anderson RC. et al,Sci Rep.,2016 6:20318.

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SKU	Supplier No.	Size	Your price
BHE10508630	CSB-E08590r	96T	$658	Add to Cart
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BHE10508630	CSB-E08590r	10x96T	$6251	Add to Cart

Rat Serum amyloid A,SAA ELISA Kit

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