Rat SerpinE2 ELISA Kit PicoKine®

Mfr.No.

EK1886

Sensitivity

<0.1 ng/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Rat

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.089	0.125	0.181	0.287	0.449	0.886	1.85	2.043

Description

Rat SerpinE2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Serpine2 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate) ,urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Glia-derived nexin; GDN; Peptidase inhibitor 7; PI-7; Protease nexin 1; PN-1; Protease nexin I; Serpin E2; Serpine2; Pi7; Pn1

Immunogen

Expression system for standard: NS0; Immunogen sequence: S20-P397

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat SerpinE2

Subcellular Localization

extracellular space

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	413	2922	9164	595	3000	9451
Standard deviation	17.35	131.49	650.64	38.68	165.00	737.18
CV (%)	4.2%	4.5%	7.1%	6.5%	5.5%	7.8%

Protein Function

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.

Background

Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 9q34. SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 aminoacid His tag atC-Terminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	413	438	416	463	432	20.08	4.6%
Sample 2	2922	2913	2827	3068	2932	86.57	2.9%
Sample 3	9164	8948	9570	8836	9129	280.31	3.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	29366
Gene Name	Serpine2
UniProt ID	P07092

Applications	ELISA
Research Area	Cardiovascular,Cell Adhesion Proteins,Cell Biology,Neurogenesis,Neurology Process,Neuroscience,Proteolysis/Ubiquitin,Proteolytic Enzymes

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001431	EK1886	96 wells/kit	$750		Add to Cart

Rat SerpinE2 ELISA Kit PicoKine®

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