Rat Serotonin Receptor 2A/5-hydroxytryptamine Receptor 2A(5-HT-2A)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14956r

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

5-hydroxytryptamine (serotonin) receptor 2A, RP11-147L20.2, 5-HT2A, HTR2, 5-HT2 receptor, 5-hydroxytryptamine receptor 2A, serotonin 5-HT-2A receptor

Recovery

The recovery of mouse D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	92-105
EDTA plasma (n=4)	97	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-98
1:200	Average %	97
	Range %	92-105
1:400	Average %	93
	Range %	87-98
1:800	Average %	95
	Range %	87-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P14842

Applications	ELISA
Research Area	HTR2A

Storage	Store at 2-8°C. Please refer to protocol.

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