Rat SEMA6A/Semaphorin-6A ELISA Kit PicoKine®

Mfr.No.

EK2057

Sensitivity

<50 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.058	0.141	0.195	0.292	0.509	0.899	1.471	2.173

Description

Rat SEMA6A ELISA Kit PicoKine® (96 Tests). Quantitate Rat Sema6a in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Semaphorin 6A; Sema6a

Immunogen

Expression system for standard: NS0; Immunogen sequence: G19-T649

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat SEMA6A

Subcellular Localization

Extracellular space. Integral component of plasma membrane.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	88	461	1323	91	471	1482
Standard deviation	3.52	26.28	60.86	4.64	32.50	103.74
CV (%)	4.0%	5.7%	4.6%	5.1%	6.9%	7.0%

Background

Semaphorin-6A is a protein that in humans is encoded by the SEMA6A gene. This gene is mapped to 18q11. Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	88	83	88	77	84	4.52	5.3%
Sample 2	461	388	441	444	433	27.35	6.3%
Sample 3	1323	1468	1499	1453	1435	67.17	4.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	361324
Gene Name	Sema6a
UniProt ID	D3ZAG0

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001569	EK2057	96 wells/kit	$499		Add to Cart

Rat SEMA6A/Semaphorin-6A ELISA Kit PicoKine®

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