Rat secretory immunoglobulin A,sIgA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08412r

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

secretory immunoglobulin A,sIgA

Recovery

The recovery of human SIRT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-103
EDTA plasma (n=4)	90	84-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SIRT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-95
1:2	Average %	92
	Range %	86-99
1:4	Average %	98
	Range %	90-105
1:8	Average %	93
	Range %	88-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	sIgA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Modulating Effect of Peptide Therapy on the Morphofunctional State of Bronchial Epithelium in Rats with Obstructive Lung Pathology. Kuzubova NA.et al,Bull Exp Biol Med.,159(5):685-8. Ethanolamine enhances intestinal functions by altering gut microbiome and mucosal anti-stress capacity in weaned rats Jian Zhou.et al,British Journal of Nutrition,May 23

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