Rat Secretogranin-3 - SCG3 ELISA Kit

Mfr.No.

E2250Ra

Sensitivity

9.43ng/L

Detection Range

20-4500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

1B1075;SCG3;Secretogranin III;Secretogranin-3;SgIII

Molecular Weight

53183

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2250Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	284.31	19.06	6.7
Intra-Assay	2	18	1484.73	51.04	3.44
Intra-Assay	3	18	792.22	40.78	5.15

Background

Member of the granin protein family that regulates the biogenesis of secretory granules (PubMed:12388744, PubMed:14597614). Acts as a sorting receptor for intragranular proteins including chromogranin A/CHGA (PubMed:12388744, PubMed:14597614). May also play a role in angiogenesis. Promotes endothelial proliferation, migration and tube formation through MEK/ERK signaling pathway (By similarity).

Protocol

These standard curves of E2250Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.475	2.542	2.488
	2.609
1200ng/L	1.457	1.474	1.42
	1.49
600ng/L	0.751	0.828	0.774
	0.904
300ng/L	0.456	0.443	0.389
	0.429
150ng/L	0.257	0.27	0.216
	0.282
0ng/L	0.061	0.053	0
	0.046

Gene ID	116635
UniProt ID	P47868

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113382	E2250Ra	48T	$320		Add to Cart
BHE12113382	E2250Ra	96T	$458		Add to Cart

Rat Secretogranin-3 - SCG3 ELISA Kit

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