Rat Secretin (Sct) ELISA kit

Mfr.No.

CSB-E17968r

Alternative Names

Secretin (Sct)

Reactivity

Rat

Detection Range

3.12 pg/mL-200 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse CLDN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	83-92
EDTA plasma (n=4)	93	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLDN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-104
1:2	Average %	95
	Range %	90-99
1:4	Average %	92
	Range %	87-98
1:8	Average %	92
	Range %	86-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

0.78 pg/mL

Limit Of Detection

450 nm

UniProt ID	P11384
Antigen	Secretin (SCT)

Applications

ELISA

Research Area

Sct

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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