Rat SDCCAG3(serologically defined colon cancer antigen 3)ELISA Kit
Alternative Names
SDCCAG3 ELISA Kit, serologically defined colon cancer antigen 3 ELISA Kit
Reactivity
Rat
Detection Method
Double Antibody
Detection Range
31.25-2000pg/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of SDCCAG3 and the recovery rates were calculated by comparing the measured value to the expected amount of SDCCAG3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-101
94
EDTA plasma(n=5)
89-101
95
heparin plasma(n=5)
88-97
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SDCCAG3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-105%
85-105%
87-101%
EDTA plasma(n=5)
83-99%
92-99%
82-101%
heparin plasma(n=5)
80-93%
84-96%
83-97%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
SDCCAG3ELISA Kit allows for the in vitro quantitative determination of SDCCAG3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
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