Rat Sclerostin - SOST ELISA Kit

Mfr.No.

E0858Ra

Sensitivity

5.12ng/L

Detection Range

10-2000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Sclerostin (also known as SOST) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Sclerostin;SOST

Molecular Weight

23974

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0858Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	122.71	7.21	5.88
Intra-Assay	2	18	677.7	26.81	3.96
Intra-Assay	3	18	406.5	17.79	4.38

Background

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

Protocol

These standard curves of E0858Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.457	2.548	2.491
	2.64
600ng/L	1.539	1.534	1.476
	1.528
300ng/L	0.931	0.876	0.819
	0.822
150ng/L	0.478	0.484	0.427
	0.491
75ng/L	0.212	0.225	0.168
	0.239
0ng/L	0.059	0.058	0
	0.057

Gene ID	80722
UniProt ID	Q99P67

Research Area	Stem Cells

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112109	E0858Ra	48T	$320		Add to Cart
BHE12112109	E0858Ra	96T	$458		Add to Cart

Rat Sclerostin - SOST ELISA Kit

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