Rat Sclerostin(SOST) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL022415RA

Sensitivity

4.68 pg/mL

Detection Range

18.75 pg/mL-1200 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

sclerosteosis, VBCH, sclerostin

Recovery

The recovery of guinea pig IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-96
EDTA plasma (n=4)	106	102-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-96
1:2	Average %	94
	Range %	91-97
1:4	Average %	103
	Range %	96-107
1:8	Average %	98
	Range %	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q99P67

Applications	ELISA
Research Area	SOST

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10508608	CSB-EL022415RA	96T	$695	Add to Cart
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BHE10508608	CSB-EL022415RA	10x96T	$6602.50	Add to Cart