Rat Sarcolipin - SLN ELISA Kit

Mfr.No.

E1270Ra

Sensitivity

0.23ng/ml

Detection Range

0.5-100ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Sarcolipin (also known as SLN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Sarcolipin;SLN

Molecular Weight

3808

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1270Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	24.8	1.37	5.5
Intra-Assay	2	18	40.3	1.09	2.7
Intra-Assay	3	18	6.8	0.45	6.6

Background

Reversibly inhibits the activity of ATP2A1 and ATP2A2 in sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in muscle. Required for muscle-based, non-shivering thermogenesis (By similarity).

Protocol

These standard curves of E1270Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.639	2.646	2.602
	2.654
32ng/ml	1.535	1.479	1.434
	1.423
16ng/ml	0.747	0.795	0.75
	0.843
8ng/ml	0.507	0.513	0.469
	0.52
4ng/ml	0.232	0.22	0.176
	0.208
0ng/ml	0.06	0.045	0
	0.03

Gene ID	367086
UniProt ID	Q6SLE7

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112513	E1270Ra	48T	$320		Add to Cart
BHE12112513	E1270Ra	96T	$458		Add to Cart

Rat Sarcolipin - SLN ELISA Kit

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