Matrices listed below were spiked with certain level of Rln3 and the recovery rates were calculated by comparing the measured value to the expected amount of Rln3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
96
EDTA plasma(n=5)
88-103
94
heparin plasma(n=5)
87-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rln3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Rln3 ELISA Kit allows for the in vitro quantitative determination of Rln3 concentrations in serum, plasma, tissue homogenates and other biological fluids.