Rat Rheumatoid Factor(RF) ELISA Kit

Mfr.No.

CSB-E13666r

Sensitivity

3.9 mIU/mL

Detection Range

15.6 mIU/mL-1000 mIU/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Rheumatoid Factor(RF)

Recovery

The recovery of pig CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	88-103
EDTA plasma (n=4)	92	84-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	103
	Range %	92-107
1:10	Average %	98
	Range %	91-102
1:20	Average %	93
	Range %	84-97
1:40	Average %	99
	Range %	90-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	RF

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Standardized ethyl acetate fraction from the roots of Brassica rapa attenuates the experimental arthritis by down regulating inflammatory responses and inhibiting NF-kB activation. Shin JS et al,Food Chem Toxicol,2014 Jan 24;66C:96-106. Ulva lactuca hydroethanolic extract suppresses experimental arthritis via its anti-inflammatory and antioxidant activities Osama M. Ahmed.et al,Beni-Suef University Journal of Basic and Applied Sciences,May 4

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