Matrices listed below were spiked with certain level of RF-IgM and the recovery rates were calculated by comparing the measured value to the expected amount of RF-IgM in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-105
95
EDTA plasma(n=5)
85-100
93
heparin plasma(n=5)
86-97
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RF-IgM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-102%
85-105%
85-99%
EDTA plasma(n=5)
86-97%
83-101%
82-98%
heparin plasma(n=5)
80-86%
85-99%
85-100%
Sensitivity
< 2.813IU/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
RF-IgM ELISA Kit allows for the in vitro quantitative determination of RF-IgM concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat RF-IgM (Rheumatoid Factor IgM) ELISA Kit