Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

Mfr.No.

CSB-E07398r

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5), D17S136E, MGC17164, RANTES, SCYA5, SISd, TCP228, OTTHUMP00000197106, SIS-delta, T-cell specific protein p288, beta-chemokine RANTES, regulated upon activation, normally T-expressed, and presumably secre

Recovery

The recovery of rat CCL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	98	93-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CCL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-98
1:2	Average %	98
	Range %	91-104
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.634	2.782	2.708	2.604
5	2.178	2.358	2.268	2.164
2.5	1.572	1.556	1.564	1.460
1.25	0.852	0.860	0.856	0.752
0.625	0.413	0.426	0.420	0.316
0.312	0.317	0.301	0.309	0.205
0.156	0.204	0.200	0.202	0.098
0	0.103	0.105	0.104	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P50231

Applications	ELISA
Research Area	RANTES, CCL5

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508581	CSB-E07398r	96T	$495	Add to Cart
BHE10508581	CSB-E07398r	5x96T	$2376	Add to Cart
BHE10508581	CSB-E07398r	10x96T	$4702.50	Add to Cart

Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

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