Rat Regucalcin - RGN ELISA Kit

Mfr.No.

E2233Ra

Sensitivity

5.24ng/L

Detection Range

10-2000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Gluconolactonase;GNL;Rc;Reguc;Regucalcin;RGN;Senescence marker protein 30;SMP-30

Molecular Weight

33390

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2233Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	668.87	30.78	4.6
Intra-Assay	2	18	937.72	29.3	3.12
Intra-Assay	3	18	254.51	11.49	4.51

Background

Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Catalyzes a key step in ascorbic acid (vitamin C) biosynthesis. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca2+ signaling, and Ca2+-dependent cellular processes and enzyme activities.

Protocol

These standard curves of E2233Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.097	2.1	2.059
	2.103
600ng/L	0.981	1.046	1.004
	1.111
300ng/L	0.435	0.476	0.435
	0.517
150ng/L	0.184	0.211	0.17
	0.239
75ng/L	0.081	0.096	0.055
	0.112
0ng/L	0.041	0.041	0
	0.041

Gene ID	25106
UniProt ID	Q03336

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113365	E2233Ra	48T	$320		Add to Cart
BHE12113365	E2233Ra	96T	$458		Add to Cart

Rat Regucalcin - RGN ELISA Kit

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