Rat Pulmonary Surfatcant-associated Protein A

Mfr.No.

E0230Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Pulmonary Surfatcant-Associated Protein A (also known as SP-A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

PSAP;PSP-A;Pulmonary surfactant-associated protein A;SFTPA 1;SFTPA1;SP-A

Reactivity

Rat

Detection Range

1-400ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

26289

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.56ng/L

Background

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.

Gene ID	24773
UniProt ID	P08427
Antigen	SFTPA1

Research Area

Signal Transduction

Protocol

These standard curves of E0230Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/L	2.128	2.186	2.113
	2.243
120ng/L	1.424	1.384	1.311
	1.344
60ng/L	0.724	0.77	0.698
	0.816
30ng/L	0.455	0.47	0.397
	0.484
15ng/L	0.247	0.257	0.184
	0.266
0ng/L	0.073	0.073	0
	0.073

Precision

We measured random samples of E0230Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	132.4	4.52	3.4
Intra-Assay	2	18	99.4	5.81	5.9
Intra-Assay	3	18	55.2	3.55	6.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111535	E0230Ra	48T	$320		Add to Cart
BHE12111535	E0230Ra	96T	$458		Add to Cart

Rat Pulmonary Surfatcant-associated Protein A - SP-A ELISA Kit

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