Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA kit

Mfr.No.

CSB-E12632r

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

surfactant protein D, COLEC7, PSP-D, SFTP4, SP-D, collectin 7, pulmonary surfactant apoprotein, pulmonary surfactant-associated protein D, surfactant, pulmonary-associated protein D, surfactant-associated protein, pulmonary

Recovery

The recovery of rat E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	101-109
EDTA plasma (n=4)	97	93-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	85-90
1:2	Average %	102
	Range %	98-106
1:4	Average %	99
	Range %	95-103
1:8	Average %	83
	Range %	80-86

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.750	2.798	2.774	2.591
100	2.045	1.968	2.007	1.824
50	1.401	1.358	1.380	1.197
25	0.920	0.967	0.944	0.761
12.5	0.576	0.598	0.587	0.404
6.25	0.445	0.468	0.457	0.274
3.12	0.307	0.312	0.310	0.127
0	0.182	0.183	0.183	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P35248

Applications	ELISA
Research Area	SFTPD

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508569	CSB-E12632r	96T	$790	Add to Cart
BHE10508569	CSB-E12632r	5x96T	$3792	Add to Cart
BHE10508569	CSB-E12632r	10x96T	$7505	Add to Cart

Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA kit

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