Rat Prothrombin - F2 ELISA Kit

Mfr.No.

E2227Ra

Alternative Names

Activation Peptide fragment 1;Activation Peptide fragment 2;Coagulation factor II;F2;Prothrombin;Thrombin heavy chain);Thrombin light chain

Reactivity

Rat

Detection Range

20-6000ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

70412

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.03ng/mL

Background

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

Gene ID	29251
UniProt ID	P18292
Antigen	F2

Research Area

Others

Protocol

These standard curves of E2227Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/mL	2.49	2.491	2.45
	2.492
1600ng/mL	1.506	1.427	1.386
	1.347
800ng/mL	0.924	0.877	0.836
	0.829
400ng/mL	0.432	0.482	0.441
	0.533
200ng/mL	0.202	0.223	0.182
	0.245
0ng/mL	0.027	0.041	0
	0.055

Precision

We measured random samples of E2227Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2027.44	56.11	2.77
Intra-Assay	2	18	895.98	43.66	4.87
Intra-Assay	3	18	637.11	44.41	6.97

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113359	E2227Ra	48T	$320		Add to Cart
BHE12113359	E2227Ra	96T	$458		Add to Cart

Rat Prothrombin - F2 ELISA Kit

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