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Home / ELISA Kit

Rat Proteoglycan 4 (PRG4) ELISA Kit

SKU : BHE13700631

Proteoglycan 4 is an approximately 345-kD proteoglycan specifically synthesized by chondrocytes located at the surface of articular cartilage, and also by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. The cDNA encodes a protein of 1,404 amino acids with a somatomedin B homology domain, heparin-binding domains, multiple mucin-like repeats, a hemopexin domain, and an aggregation domain. There are 3 consensus sequences for N-glycosylation and 1 chondroitin sulfate substitution site. The locus for autosomal recessive camptodactyly-arthropathy-coxa vara-pericarditis syndrome maps to chromosome 1q25-q31. This protein may act as a joint/intimal cell lubricant, and cell overgrowth may be primary to the pathogenesis of this protein.

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Mfr.No.	KTE100441
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Rat Proteoglycan 4 (PRG4) ELISA Kit employs a two-site sandwich ELISA to quantitate PRG4.
Alternative Names	PRG4; GS1-174L6.2; CACP; FLJ32635; HAPO; JCAP; MSF; SZP; bG174L6.2; OTTHUMP00000033580; articular superficial zone protein; bG174L6.2 (MSF: megakaryocyte stimulating factor ); lubricin; megakaryocyte stimulat
Detection Method	Colorimetric
Kit Components	Rat Proteoglycan 4 microplate Rat Proteoglycan 4 standard Rat Proteoglycan 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat Proteoglycan 4 (PRG4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRG4. No significant cross-reactivity or interference between Rat PRG4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Proteoglycan 4 is an approximately 345-kD proteoglycan specifically synthesized by chondrocytes located at the surface of articular cartilage, and also by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. The cDNA encodes a protein of 1,404 amino acids with a somatomedin B homology domain, heparin-binding domains, multiple mucin-like repeats, a hemopexin domain, and an aggregation domain. There are 3 consensus sequences for N-glycosylation and 1 chondroitin sulfate substitution site. The locus for autosomal recessive camptodactyly-arthropathy-coxa vara-pericarditis syndrome maps to chromosome 1q25-q31. This protein may act as a joint/intimal cell lubricant, and cell overgrowth may be primary to the pathogenesis of this protein.
Notes	This Rat Proteoglycan 4 (PRG4) ELISA Kit employs a two-site sandwich ELISA to quantitate PRG4 in samples. An antibody specific for PRG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10216

Applications	ELISA
Applications Notes	This Rat Proteoglycan 4 (PRG4) ELISA Kit employs a two-site sandwich ELISA to quantitate PRG4 in samples. An antibody specific for PRG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700631	KTE100441	48 T	$339		Add to Cart
BHE13700631	KTE100441	96 T	$589		Add to Cart
BHE13700631	KTE100441	96 Tx5	$2629		Add to Cart
BHE13700631	KTE100441	96 Tx50	$24569		Add to Cart

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