Rat prostate specific antigen,PSA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08275r

Sensitivity

0.195 pg/mL

Detection Range

0.78 pg/mL-50 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

prostate specific antigen,PSA

Recovery

The recovery of hamster IL-1? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-1? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	105
	Range %	100-112
1:4	Average %	89
	Range %	84-93
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PSA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Sphaeranthus indicus attenuates testosterone induced prostatic hypertrophy in albino rats Alok Nahata et al,Phytotherapy Research,2011 Dec;25(12):1839-48. Ganoderma lucidum is an inhibitor of testosterone-induced prostatic hyperplasia in rats A. Nahata et al,Andrologia,2012 May;44 Suppl 1:160-74. Ameliorative effects of stinging nettle (Urtica dioica) on testosterone-induced prostatic hyperplasia in rats A. Nahata et al,Andrologia,2012 May;44 Suppl 1:396-409. Action Mechanism of Ginkgo biloba Leaf Extract Intervened by Exercise Therapy In Treatment of Benign Prostate Hyperplasia Peng CC et al,Evid Based Complement Alternat Med,2013:408734. Preventive effect of Pueraria mirifica on testosterone-induced prostatic hyperplasia in Sprague Dawley rats Masrudin SS.et al,Andrologia.,47(10):1153-9 Corn silk extract improves benign prostatic hyperplasia in experimental rat model Kim SR.et al,NUTR RES PRACT,Oct Cynanchum wilfordii Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia by Regulating 5a-Reductase and Androgen Receptor Activities in a Rat Model Lee G.et al,Nutrients,Sep 27 Nano-sized titanium dioxide toxicity in rat prostate and testis: Possible ameliorative effect of Morin Shahin NN.et al,Toxicol Appl Pharmacol,Aug 24

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