Rat Prostaglandin-H2 D-isomerase(PTGDS) ELISA kit

Rat Prostaglandin-H2 D-isomerase(PTGDS) ELISA kit

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Mfr.No.

CSB-EL018969RA

Alternative Names

prostaglandin D2 synthase 21kDa (brain), RP11-229P13.6, L-PGDS, LPGDS, PDS, PGD2, PGDS, PGDS2, PGD2 synthase, beta-trace protein, glutathione-independent PGD synthase, lipocalin-type prostaglandin D synthase, prostaglandin D synthase, prostagla

Reactivity

Rat

Detection Range

9.37 ng/mL-600 ng/mL

Sample Type

Serum, Plasma, Cerebrospinal fluid, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human IKZF3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	96	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IKZF3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-91
1:2	Average %	96
	Range %	91-104
1:4	Average %	96
	Range %	92-109
1:8	Average %	94
	Range %	86-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.098	1.986	2.042	1.950
800	1.357	1.298	1.328	1.236
400	0.825	0.789	0.807	0.715
200	0.500	0.481	0.491	0.399
100	0.360	0.351	0.356	0.264
50	0.245	0.240	0.243	0.151
25	0.156	0.152	0.154	0.062
0	0.092	0.091	0.092	?

Species

Rattus norvegicus

Sensitivity

2.34 ng/mL

Limit Of Detection

450 nm

UniProt ID	P22057
Antigen	prostaglandin D2 synthase 21kDa (brain)

Applications

ELISA

Research Area

PTGDS

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508537	CSB-EL018969RA	96T	$695	Add to Cart
BHE10508537	CSB-EL018969RA	5x96T	$3336	Add to Cart
BHE10508537	CSB-EL018969RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.