Rat prostacycline,PGI2 ELISA Kit

Rat prostacycline,PGI2 ELISA Kit

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Mfr.No.

CSB-E13706r

Alternative Names

prostacycline,PGI2

Reactivity

Rat

Detection Range

0.25 ng/mL-50 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of rabbit COL ? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-92
EDTA plasma (n=4)	93	87-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit COL ? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-91
1:2	Average %	93
	Range %	89-97
1:4	Average %	98
	Range %	95-104
1:8	Average %	86
	Range %	80-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	2.734	2.534	2.634	2.541
400	2.055	2.135	2.095	2.002
200	1.348	1.324	1.336	1.243
100	0.677	0.669	0.673	0.580
50	0.412	0.421	0.417	0.324
25	0.278	0.274	0.276	0.183
12.5	0.151	0.154	0.153	0.060
0	0.093	0.092	0.093

Species

Rattus norvegicus

Sensitivity

0.125 ng/mL

Limit Of Detection

450 nm

Antigen	prostacycline,PGI2

Applications

ELISA

Research Area

PGI2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	VASOCONSTRICTIVE EFFECT OF XINMAILONG INJECTION IN RAT AORTA Cui Yang.et al,Afr J Tradit Complement Altern Med,/ The Effects of 17-Methoxyl-7-Hydroxy-Benzene-Furanchalcone on Pressure Overload-Induced Cardiac Remodeling in Rats and the Endothelial Mechanisms Based on PGI2 Huang J.. et al,Cell Physiol Biochem,2015 36(3):1004-14 Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats Mohammad Afiq Hamsi et al,Alexandria Journal of Medicine,2015 Mar;51(1):53-63

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SKU	Supplier No.	Size	Your price
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BHE10508534	CSB-E13706r	5x96T	$3792	Add to Cart
BHE10508534	CSB-E13706r	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.