Rat Pro-opiomelanocortin(POMC) ELISA KIT

Mfr.No.

CSB-E13569r

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

proopiomelanocortin, ACTH, CLIP, LPH, MSH, NPP, POC, OTTHUMP00000119991, OTTHUMP00000200964, adrenocorticotropic hormone, adrenocorticotropin, alpha-MSH, alpha-melanocyte-stimulating hormone, beta-LPH, beta-MSH, beta-endorphin,, proopiomelanocortin, ACTH, CLIP, LPH, MSH, NPP, POC, OTTHUMP00000119991, OTTHUMP00000200964, adrenocorticotropic hormone, adrenocorticotropin, alpha-MSH, alpha-melanocyte-stimulating hormone, beta-LPH, beta-MSH, beta-endorphin,

Recovery

The recovery of human COL4A3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	99-105
EDTA plasma (n=4)	96	92-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human COL4A3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	104
	Range %	101-107
1:10	Average %	91
	Range %	88-98
1:20	Average %	96
	Range %	91-103
1:40	Average %	89
	Range %	86-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.544	2.511	2.528	2.347
5	2.071	2.023	2.047	1.866
2.5	1.356	1.458	1.407	1.226
1.25	0.776	0.774	0.775	0.594
0.625	0.450	0.488	0.469	0.288
0.312	0.292	0.271	0.282	0.101
0.156	0.208	0.220	0.214	0.033
0	0.178	0.184	0.181	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P01194

Applications	ELISA
Research Area	POMC

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508532	CSB-E13569r	96T	$790	Add to Cart
BHE10508532	CSB-E13569r	5x96T	$3792	Add to Cart
BHE10508532	CSB-E13569r	10x96T	$7505	Add to Cart

Rat Pro-opiomelanocortin(POMC) ELISA KIT

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