Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit

Mfr.No.

ELK8404

Sensitivity

16.4 pg/mL

Detection Range

62.5-4000 pg/mL

Reactivity

Rat

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

pro-IL1-B; pro-IL1-Beta; pro-IL1F2; pro-IL-1beta

Recovery

Matrices listed below were spiked with certain level of recombinant pro-IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of pro-IL1b in samples.

Matrix	Recovery range	Average
serum(n=5)	89-107%	98%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	78-90%	84%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pro-IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	89-97%	85-94%	79-96%	85-99%
EDTA plasma(n=5)	88-97%	86-92%	81-93%	89-105%
Heparin plasma(n=5)	85-94%	79-96%	92-101%	83-90%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
4000.00	1.978	1.874
2000.00	1.572	1.468
1000.00	0.996	0.892
500.00	0.821	0.717
250.00	0.539	0.435
125.00	0.308	0.204
62.50	0.252	0.148
0.00	0.104	0.000

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

4000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Cytokine;Tumor immunity;Infection immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15204422	ELK8404	48T	$320		Add to Cart
BHE15204422	ELK8404	96T	$458		Add to Cart

Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit

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