Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Pro-ADM and the recovery rates were calculated by comparing the measured value to the expected amount of Pro-ADM in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
97
EDTA plasma(n=5)
94-101
98
heparin plasma(n=5)
86-102
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pro-ADM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
91-98%
85-96%
EDTA plasma(n=5)
83-101%
83-100%
85-100%
heparin plasma(n=5)
80-83%
83-98%
84-99%
Sensitivity
< 0.938pmol/l
Notes
For Research Use Only
UniProt ID
P43145
Antigen
Pro-ADM
Applications
ELISA
Applications Notes
Pro-ADM ELISA Kit allows for the in vitro quantitative determination of Pro-ADM concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Pro-ADM (Proadrenomedullin) ELISA Kit