Matrices listed below were spiked with certain level of Prkab1 and the recovery rates were calculated by comparing the measured value to the expected amount of Prkab1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
94
EDTA plasma(n=5)
85-103
95
heparin plasma(n=5)
88-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prkab1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Prkab1/PRKAB1/5'-AMP-activated protein kinase subunit beta-1/AMPK subunit beta-1/AMPKb/AMP
Notes
For Research Use Only
UniProt ID
P80386
Antigen
PRKAB1
Applications
ELISA
Applications Notes
Prkab1 ELISA Kit allows for the in vitro quantitative determination of Prkab1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Prkab1(5 -AMP-activated protein kinase subunit beta-1) ELISA Kit