Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Ppat and the recovery rates were calculated by comparing the measured value to the expected amount of Ppat in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
92
EDTA plasma(n=5)
87-105
98
heparin plasma(n=5)
88-104
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ppat and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-100%
85-103%
89-105%
EDTA plasma(n=5)
86-100%
92-101%
85-95%
heparin plasma(n=5)
80-96%
84-99%
84-96%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P35433
Applications
ELISA
Applications Notes
Ppat ELISA Kit allows for the in vitro quantitative determination of Ppat concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Ppat (Amidophosphoribosyltransferase) ELISA Kit