Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of PPAR-alpha and the recovery rates were calculated by comparing the measured value to the expected amount of PPAR-alpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-104
98
EDTA plasma(n=5)
85-104
93
heparin plasma(n=5)
89-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PPAR-alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PPAR-alpha ELISA Kit allows for the in vitro quantitative determination of PPAR-alpha concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat PPAR-alpha (Peroxisome ProlifeRators Activator Receptors alpha) ELISA Kit