Matrices listed below were spiked with certain level of POMC and the recovery rates were calculated by comparing the measured value to the expected amount of POMC in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
97
EDTA plasma(n=5)
87-95
92
heparin plasma(n=5)
85-105
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of POMC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
96-104%
85-104%
90-103%
EDTA plasma(n=5)
89-101%
86-97%
89-101%
heparin plasma(n=5)
85-99%
85-99%
94-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P01194
Applications
ELISA
Applications Notes
POMC ELISA Kit allows for the in vitro quantitative determination of POMC concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat POMC(Proopiomelanocortin) ELISA Kit