Rat Pola2(DNA polymerase alpha subunit B) ELISA Kit

Mfr.No.

ER0533

Alternative Names

Pola2 ELISA Kit, DNA polymerase alpha subunit B ELISA Kit, DNA polymerase alpha 70 kDa subunit ELISA Kit, DNA polymerase subunit II ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

31.25-2000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Pola2 and the recovery rates were calculated by comparing the measured value to the expected amount of Pola2 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	94-97	95
EDTA plasma(n=5)	95-104	98
heparin plasma(n=5)	86-105	96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pola2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	86-99%	87-105%	85-105%
EDTA plasma(n=5)	84-93%	85-100%	86-95%
heparin plasma(n=5)	84-100%	84-99%	84-100%

Sensitivity

< 18.75pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Pola2/DNA polymerase alpha subunit B/DNA polymerase alpha 70 kDa subunit/DNA polymerase subunit II

Notes

For Research Use Only

UniProt ID	O89043
Antigen	POLA2

Applications	ELISA
Applications Notes	Pola2 ELISA Kit allows for the in vitro quantitative determination of Pola2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10802894	ER0533	96T	$400		Add to Cart

Rat Pola2(DNA polymerase alpha subunit B) ELISA Kit

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