Rat Podocin - NPHS2 ELISA Kit

Mfr.No.

E1595Ra

Sensitivity

0.092ng/mL

Detection Range

0.2-70ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Podocin (also known as NPHS2) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

NPHS 2;NPHS2;Podocin

Molecular Weight

42387

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1595Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9	0.64	7.1
Intra-Assay	2	18	12.5	0.68	5.4
Intra-Assay	3	18	27.5	1.15	4.2

Background

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

Protocol

These standard curves of E1595Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/mL	2.197	2.176	2.108
	2.155
20ng/mL	1.436	1.375	1.307
	1.314
10ng/mL	0.805	0.806	0.738
	0.807
5ng/mL	0.445	0.491	0.423
	0.537
2.5ng/mL	0.232	0.281	0.212
	0.329
0ng/mL	0.074	0.068	0
	0.062

Gene ID	170672
UniProt ID	Q8K4G9

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112813	E1595Ra	48T	$320		Add to Cart
BHE12112813	E1595Ra	96T	$458		Add to Cart

Rat Podocin - NPHS2 ELISA Kit

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