Rat Plexin B2/PLXNB2 ELISA Kit PicoKine®

Mfr.No.

EK1883

Sensitivity

<50 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Rat

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.126	0.183	0.235	0.344	0.536	0.917	1.513	2.230

Description

Rat Plexin B2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Plxnb2 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Plexin B2; Plxnb2; rCG_59297

Immunogen

Expression system for standard: CHO; Immunogen sequence: L20-P1202

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat Plexin B2

Subcellular Localization

integral component of plasma membrane

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	31	190	449	33	201	456
Standard deviation	1.89	10.26	19.76	1.55	14.67	35.11
CV (%)	6.1%	5.4%	4.4%	4.7%	7.3%	7.7%

Background

Plexin-B2 is a protein that in humans is encoded by the PLXNB2 gene. It is mapped to 7q34. Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	31	33	35	36	33	1.92	5.8%
Sample 2	190	161	186	179	179	11.11	6.2%
Sample 3	449	432	432	456	442	10.54	2.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	315217
Gene Name	Plxnb2
UniProt ID	D3ZQ57

Applications	ELISA
Research Area	Axonal Guidance Proteins,Cell Type Marker,Growth and Development,Neurogenesis,Neurology Process,Neuron Marker,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001429	EK1883	96 wells/kit	$499		Add to Cart

Rat Plexin B2/PLXNB2 ELISA Kit PicoKine®

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