Rat Plexin A1/PLXNA1 ELISA Kit PicoKine®

Mfr.No.

EK1819

Sensitivity

<0.1 ng/ml

Detection Range

0.312 ng/ml - 20 ng/ml

Reactivity

Rat

Sample Volume

Concentration (ng/ml)	0	0.3	0.63	1.25	2.5	5	10	20
O.D.	0.158	0.232	0.286	0.326	0.607	0.935	1.409	2.343

Description

Rat Plexin A1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Plxna1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Plexin A1; RCG56095; Plxna1; RGD1560871; rCG_56095

Immunogen

Expression system for standard: NS0; Immunogen sequence: S46-S1255

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat Plexin A1

Subcellular Localization

integral component of membrane

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	525	2481	8381	569	2489	9641
Standard deviation	28.35	151.34	645.34	27.88	136.90	655.59
CV (%)	5.4%	6.1%	7.7%	4.9%	5.5%	6.8%

Background

Plexin-A1 is a protein that in humans is encoded by the PLXNA1 gene. This gene is mapped to 4q34. Plexin A1 has been shown to interact with AKT1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	525	574	531	506	534	24.86	4.6%
Sample 2	2481	2577	2752	2419	2557	125.74	4.9%
Sample 3	8381	9525	9678	8669	9063	550.46	6.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	362398
Gene Name	Plxna1
UniProt ID	D3Z981

Applications	ELISA
Research Area	Axonal Guidance Proteins,Growth and Development,More Gpcr,Neurogenesis,Neurology Process,Neuroscience,Neurotransmission,Receptors / Channels

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001420	EK1819	96 wells/kit	$750		Add to Cart

Rat Plexin A1/PLXNA1 ELISA Kit PicoKine®

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