Rat plasminogen activator inhibitor 1,PAI1 ELISA Kit

Rat plasminogen activator inhibitor 1,PAI1 ELISA Kit

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Mfr.No.

CSB-E07948r

Sensitivity

19.5 pg/ml

Detection Range

78 pg/ml-5000 pg/ml

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, PAI, PAI-1, PAI1, PLANH1, plasminogen activator inhibitor-1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

Recovery

The recovery of human WNT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	88	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human WNT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	97
	Range %	93-101
1:4	Average %	105
	Range %	98-109
1:8	Average %	92
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.524	2.634	2.579	2.466
15	1.896	1.756	1.826	1.713
7.5	1.181	1.162	1.172	1.059
3.75	0.719	0.738	0.729	0.616
1.8	0.409	0.424	0.417	0.304
0.9	0.297	0.286	0.292	0.179
0.45	0.210	0.215	0.213	0.100
0	0.112	0.113	0.113

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P20961

Applications	ELISA
Research Area	SERPINE1

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.