Rat placenta growth factor,PLGF ELISA kit

Mfr.No.

CSB-E07400r

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

placental growth factor, D12S1900, PGFL, PLGF, PlGF-2, SHGC-10760, placenta growth factor, placental growth factor, vascular endothelial growth factor-related protein, placental growth factor-like

Recovery

The recovery of human RSPO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-98
EDTA plasma (n=4)	113	108-117

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human RSPO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-104
1:2	Average %	91
	Range %	82-96
1:4	Average %	103
	Range %	98-109
1:8	Average %	87
	Range %	82-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.162	2.312	2.237	2.121
2000	1.654	1.745	1.700	1.584
1000	1.122	1.212	1.167	1.051
500	0.669	0.698	0.684	0.568
250	0.452	0.464	0.458	0.342
125	0.312	0.322	0.317	0.201
62.5	0.223	0.213	0.218	0.102
0	0.112	0.120	0.116	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q63434

Applications	ELISA
Research Area	PGF

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508489	CSB-E07400r	96T	$615	Add to Cart
BHE10508489	CSB-E07400r	5x96T	$2952	Add to Cart
BHE10508489	CSB-E07400r	10x96T	$5842.50	Add to Cart

Rat placenta growth factor,PLGF ELISA kit

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