Rat Pkm2(Pyruvate kinase isozymes M1/M2) ELISA Kit

Mfr.No.

ER0329

Alternative Names

Pkm2 ELISA Kit, Pykm ELISA Kit, Pykm ELISA Kit, Pyruvate kinase isozymes M1 ELISA Kit, M2 ELISA Kit, Pyruvate kinase muscle isozyme ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

1.25-80IU/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Pkm2 and the recovery rates were calculated by comparing the measured value to the expected amount of Pkm2 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	89-100	94
EDTA plasma(n=5)	86-105	92
heparin plasma(n=5)	87-105	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pkm2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-105%	86-102%	92-105%
EDTA plasma(n=5)	87-101%	84-100%	83-100%
heparin plasma(n=5)	92-100%	81-100%	83-97%

Sensitivity

< 0.75IU/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Pkm2/Pykm/Pykm/Pyruvate kinase isozymes M1/M2/Pyruvate kinase muscle isozyme

Notes

For Research Use Only

UniProt ID	P11980
Antigen	PKM2

Applications	ELISA
Applications Notes	Pkm2 ELISA Kit allows for the in vitro quantitative determination of Pkm2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10802652	ER0329	96T	$400		Add to Cart

Rat Pkm2 (Pyruvate kinase isozymes M1/M2) ELISA Kit

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