Rat Pituitary Adenylate Cyclase-activating Polypeptide

Mfr.No.

E2197Ra

Alternative Names

ADCYAP 1;ADCYAP1;PACAP;PACAP27;PACAP-27;PACAP38;PACAP-38;PACAP-related Peptide;Pituitary adenylate cyclase-activating polypeptide;Pituitary adenylate cyclase-activating polypeptide 27;Pituitary adenylate cyclase-activating polypeptide 38;PRP-48

Reactivity

Rat

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

19557

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.32ng/L

Background

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells (By similarity). Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway. In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (PubMed: 18198219). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells (By similarity).

Gene ID	24166
UniProt ID	P13589
Antigen	ADCYAP1

Research Area

Neuroscience

Protocol

These standard curves of E2197Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.247	2.156	2.096
	2.065
600ng/L	1.463	1.421	1.361
	1.379
300ng/L	0.732	0.756	0.697
	0.781
150ng/L	0.509	0.455	0.395
	0.401
75ng/L	0.24	0.267	0.207
	0.294
0ng/L	0.048	0.06	0
	0.071

Precision

We measured random samples of E2197Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	164.8	8.71	5.3
Intra-Assay	2	18	333.8	15.61	4.7
Intra-Assay	3	18	607.8	22.18	3.6

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12113329	E2197Ra	48T	$320		Add to Cart
BHE12113329	E2197Ra	96T	$458		Add to Cart

Rat Pituitary Adenylate Cyclase-activating Polypeptide - ADCYAP1 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat Pituitary Adenylate Cyclase-activating Polypeptide - ADCYAP1 ELISA Kit

Rate it now..!!