Matrices listed below were spiked with certain level of PIIINT and the recovery rates were calculated by comparing the measured value to the expected amount of PIIINT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
94-103
96
EDTA plasma(n=5)
86-103
94
heparin plasma(n=5)
89-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIIINT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PIIINPELISA Kit allows for the in vitro quantitative determination of PIIINT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat PIIINP(N-terminal propeptide of procollagen type III)ELISA Kit