Rat pigment epithelium-derived factor,PEDF ELISA Kit

Rat pigment epithelium-derived factor,PEDF ELISA Kit

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Mfr.No.

CSB-E08819r

Sensitivity

1.56 pg/mL

Detection Range

6.25 pg/mL-400 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

pigment epithelium-derived factor,PEDF

Recovery

The recovery of mouse Col ? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-93
EDTA plasma (n=4)	104	95-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col ? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-92
1:2	Average %	101
	Range %	94-105
1:4	Average %	90
	Range %	85-94
1:8	Average %	97
	Range %	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
120	2.668	2.511	2.590	2.401
60	2.149	2.100	2.125	1.936
30	1.459	1.445	1.452	1.263
15	0.858	0.832	0.845	0.656
7.5	0.540	0.555	0.548	0.359
3.75	0.383	0.389	0.386	0.197
1.87	0.292	0.298	0.295	0.106
0	0.188	0.190	0.189

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q80ZA3

Applications	ELISA
Research Area	PEDF

Storage	Store at 2-8°C. Please refer to protocol.

Reference	PEDF and PEDF-derived peptide 44mer stimulate cardiac triglyceride degradation via ATGL Gao X et al,Sci Rep,2014 Jul 11;4:5637. Comparison of the effects of letrozole and cabergoline on vascular permeability, ovarian diameter, ovarian tissue VEGF levels, and blood PEDF levels, in a rat model of ovarian hyperstimulation syndrome. ahin N. et al,Arch Gynecol Obstet,2015 Dec 21 Influence of triazine herbicide exposure on guppies (poecilia sphenops) aromatase activities, altered sex steroid concentration and vitellogenin induction S Vasanth. et al,Indian J Pharm Sci.,2015 Mar-Apr;77(2):156-62. PEDF Improves Cardiac Function in Rats with Acute Myocardial Infarction via Inhibiting Vascular Permeability and Cardiomyocyte Apoptosis Zhang H et al,Int J Mol Sci,2015 Mar 11;16(3):5618-34. PEDF and 34-mer inhibit angiogenesis in the heart by inducing tip cells apoptosis via up-regulating PPAR-r to increase surface FasL Zhang H. et al,Apoptosis,2015 online Pigment Epithelium-Derived Factor (PEDF) Improves Ischemic Cardiac Functional Reserve ThroughDecreasing Hypoxic Cardiomyocyte Contractility Through PEDF Receptor (PEDF-R). Lu P.et al,J Am Heart Assoc.,5(7).pii: e003179. PEDF and PEDF-derived peptide 44mer inhibit oxygen-glucose deprivation-induced oxidative stress through upregulating PPARr via PEDF-R in H9c2 cells. Wei Zhuang.et al,Biochem Biophys Res Commun.,2016 472(3):482-8. A novel modified physiologically relevant model for cardiac angiogenesis Hao Zhang.et al,Microvasc Res,Jun 28 Pigment epithelium-derived factor attenuates myocardial fibrosis via inhibiting Endothelial-to-Mesenchymal Transition in rats with acute myocardial infarction Hao Zhang.et al,Scientific Reports,Feb 7 Application of rat cryptorchidism model for the evaluation of mesenchymal stromal cell secretome regenerative potential G.D.Sagaradze, et al,Biomedicine & Pharmacotherapy,November 13

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.